WebChromophore assisted laser inactivation (CALl) is a new means of addressing functional interactions in biology with a high degree of spatial and temporal resolution. CALl is analogous to cellular laser ablation but the damage is localized to a protein of interest by covalently attaching a WebMar 29, 1994 · Chromophore-assisted laser inactivation (CALI) is a technique that selectively inactivates proteins of interest to elucidate their in vivo functions. This method has application to a wide array of biological questions and an understanding of …
Laser-Mediated, Site-Specific Inactivation of RNA …
WebAbstract. Differential protein regulation is a critical biological process that regulates cellular activity and controls cell fate determination. It is especially important during early embryogenesis when post-transcriptional events predominate differential fate specification in many organisms. Light-induced approaches have been a powerful ... Webnotype. Chromophore-assisted laser inactivation using mal-achite green (MG)-tagged antibodies makes it possible to inactivate target proteins in a highly restricted manner, probing their temporally and spatially resolved functions. In this report, we describe the isolation and in vitro character-ization of a MG-binding RNA motif that may enable ... small homes wyoming builders
Chromophore-assisted Laser Inactivation of Alpha- And Gamma …
WebThe present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, WebMar 1, 2002 · Chromophore‐assisted laser inactivation (CALI) provides a timely and locally restricted protein inactivation and has proven to specifically destroy protein function using dye‐coupled ligands and laser irradiation. CALI involves the generation of short‐lived radicals thus limiting the radius of covalent modifications to spatially ... WebLaser microirradiation of kinetochores in mitotic PtK2 ... Chromophore-assisted laser inactivation (CALI) to elucidate cellular mecha-nisms of cancer. Biochimica et Biophysica Acta,vol. small homes with wrap around porches