Irf3 knockout mice
WebJun 5, 2024 · The IRF3 knockout (KO) mice develop obesity, insulin resistance, glucose intolerance, and eventually type 2 diabetes with aging, which is associated with the … WebNov 2, 2024 · To identify potential IRF3-associated lncRNAs in vivo, we first generated the genetic manipulated mice with a FLAG-tag sequence inserted at the 3′ end of the coding sequence of Irf3 genes (Irf3 flag/flag) to facilitate the purification of endogenous IRF3 protein.Then we performed FA-CLIP with anti-FLAG antibody in primary mouse peritoneal …
Irf3 knockout mice
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WebIRF-3(-/-) and select IRF-3/7(-/-) mice were resistant to LP-BM5-induced pathogenesis. However, further analyses strongly suggested that resistance could be attributed to strain … WebMay 21, 2024 · To investigate the role of IRF7 in adipocytes, we examined gene expression patterns in 3T3-L1 cells infected with a retrovirus carrying the IRF7 gene and found that enforced IRF7 expression induced the expression of monocyte chemoattractant protein-1 (MCP-1), a key initial adipokine in the chronic inflammation of obesity.
WebThe IRF3 conditional knockout mouse may be a useful tool to identify cell type-specific functions of this transcription factor. Abstract IFN regulatory factor 3 (IRF3) is a … WebGEO help: Mouse over screen elements for information. Scope: Format: Amount: GEO accession: Series GSE209654: Query DataSets for GSE209654: Status: Public on Apr 14, …
WebAged WT and IRF3-KO mice were subject to CLP to induce sepsis, and a second group of aged WT mice were subject to sham surgery as a control. Graphs show pooled male and female data (n=10/group CLP, n=5/group sham) for (A) serum IL-6, (B) serum IL-12/23p40, (C) serum MCP-1. This cohort was split into male and female subgroups to show the … WebIRF3 Inhibits Virus-Induced Inflammatory Gene Induction in Mouse Macrophages. To investigate the cellular mechanism of IRF3-mediated regulation of inflammatory …
WebSep 23, 2010 · We show in knockout mice that Irf3 deficiency causes severe tissue pathology and that effector functions controlled by IFNβ are involved. Finally, in highly disease-prone pyelonephritis patients we found a high frequency of IRF3 promoter polymorphism compared to asymptomatic bacterial carriers or controls.
WebNov 4, 2024 · Recent studies have suggested that STING signaling is necessary for the anticancer immune response based on the following observations: on the one hand, STING knockout mice and IRF3 knockout mice show impaired spontaneous T-cell responses against tumors [34, 35]; on the other hand, STING agonists show a favorable effect in … cvs pinhook and south parkWebSee Taconic Bioscience’s Constitutive Knock Out mouse, model # Gpr183. Toggle navigation. ... For most lines, the mouse ES cells used in generating the model are of the … cheap flights from male to dhakaWebNov 13, 2024 · IRF3-knockout cells were generated in human colon cell line HCT116 and SW620, as well as human non-small lung carcinoma cell line H1299, as Wnt signaling is … cvs pinhook lafayette laWebMar 4, 2024 · Knockdown of USP22 inhibits IRF3 nuclear accumulation in human cell lines To determine whether USP22 is a physiological regulator for IRF3, we designed three siRNAs targeting USP22, two of which potently down-regulated the protein levels of ectopic and endogenous USP22 ( Fig. 2 A ). cheap flights from malta to milanWebNov 7, 2024 · IRF3 is a downstream molecule of the cGAS/STING signaling pathway, but its roles have not been investigated in MESO. Methods: Various murine mesothelioma cell lines were inoculated into wild type (WT) and IRF3 knockout … cheap flights from male\u0027 to kulWebAdditionally, TAT-PAMAM/pBud-H5-GFP-IRF3 vaccination increased the level of IL-2, IL-15, IL-12, IL-6, and TNF-α cytokines, which are also able to enhance the IFN-γ production. Studies conducted in IL-12 knockout mice have shown that IL-12 is essential in enhancing IFN-γ gene expression following endotoxin administration. cheap flights from malta to bucharestWebWild-type and IRF3 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween ® ) before incubation with ab76409 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. cvs pitcher